Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.
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Table of Contents Alerts. Several herbs and plants activiyy diversified pharmacological properties have been shown to have potential to prevent human cancers as they possess antitumor substances which have the potential to cure cancer without causing toxicity. The Graphpad Prism software version 5 was used for drawing the graphs.
A pilot double blind study. The preliminary examination was done by observing the confluency of the test as well as control samples which showed cell growth inhibition in the test samples Fig.
BioMed Research International
Variance analysis of salinity effects on morphological traits, proline, activitty the three main phytochemicals in A. The effects of salinity levels on studied traits among the four accessions of A. Since metabolic adaptations towards environmental stress including salinity affect global metabolic fluxes, some beneficial secondary metabolites of therapeutic importance or so-called phytochemicals would also be affected [ 11 ].
The herb exhibited a wide scope of pharmaceutical properties such as anti-HIV [ 19 ], anti-H1N1 [ 20 ], anticancer [ 21 ], antihepatitis [ 22 ], anti-inflammatory [ 23 ], blood purifier, and antidiarrhea [ 24 ].
In conclusions, the result supports the use of the plant as described in folk medicine, that the aerial plant parts can be used to treat cancer. Thus, consuming a diet rich in antioxidant plant material can provide health-protective effects.
The analysis of variance showed that different levels of salinity affected the proline and the three main phytochemicals of A. After 30 days of culturing almost in 70 days oldthe plants were placed in different salinity levels.
The seeds were germinated as described by Talei et al. The human tumor cytotoxicity was determined by the protocols established by NCI Monks et al. HDACs are the epigenetic manipulators which plays an important role in the gene silencing which leads to the initiation of cancer and rapid proliferation.
Protective effects of silymarin against photocarcinogenesis in a andrographus skin annticancer. Again, the combination 1: Likewise, many researchers suggested that proline and soluble flavonoids are involved in osmotic adjustment, protecting the cellular structures from oxidative damage and osmotic stress by accumulation in the vacuole, and so play an important role in increasing the oxidative stress tolerance through protecting the chloroplast and photosynthetic systems versus solar radiation by absorbing UV [ 2829 ].
The decreasing trend of all studied morphological traits was linear Table 2. The trend of proline and the three main phytochemicals in tolerant accession were linear and positive, while in sensitive accession they were nonlinear.
The combination of both the plants 1: Interestingly, the correlation between proline as well as NAG with the other measured morphological characteristics was significant and positive in the salt-stressed plants.
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The process of programmed cell death is called apoptosis. Phenols, polyphenols, flavonoids and their derivatives are ubiquitous in plants and have been found associated with the inhibition of atherosclerosis and cancer Cirla and Mann, The plant extract contains three major groups of phytochemical compounds, namely, diterpenes, flavonoids and stigmasterols [ 16 ].
These salinities were applied using In spite of the mentioned trends, the differences between those two accessions were not statistically significant [ 36 ]. Since the highest amount of the active components is found just before the plant blooms [ 39 ], the day-old plants were subjected to different salinity levels. Many of those metabolic changes such as sucrose, oligosaccharides, polyhydric alcohols, proline, and polyamines are observable in the leaves of plants exposed to abiotic stresses [ 4 ].
Off suspensions were prepared by the treatment of cells with 0.
The present study aimed antifancer investigate the potential of salt stress to enhance the accumulation of the anticancer phytochemicals in Andrographis paniculata accessions.
The dry weight was used to monitor the physiological response of each accession to salinity. Abstract Salinity causes the adverse effects in all physiological processes of plants.
Hepatoprotective and immunological effects of antioxidant drugs. Effect of silymarin on different acute inflammation models and androgralhis leukocyte migration. The filtrate was evaporated to dryness using a rotary evaporator yield was Enhancement of phenolic and flavonoid compounds in onion plant under salinity stress has been reported to improve the deleterious effect of salinity stress [ 34 ].
Gour Central University, Sagar Activitt. Generally, plants produce secondary metabolites in nature as defense mechanisms under different environmental stresses [ 27 ]. Our results indicated a positive correlation between the three anticancer phytochemicals and proline accumulation, which might be closely related to tolerance abilities indicated by physiological performances.
The correlative aspects of phytochemical production as well as the accumulation of proline in salt-tolerant a and salt-sensitive b A.
Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress
However, to prove their efficacy for clinical utilization more scientific data are needed. One mL of each standard sample was finally filtered into HPLC vials using the same syringe filters in five replicates [ 4041 ].
The germinated seeds at two initial leaf stages were transferred into the Jiffy media. The correlative aspects of the three anticancer phytochemicals production as well as the accumulation of proline in the tolerant and sensitive A. Apart from this, alkaloids were present only in the extract of Andrographis paniculata while, saponins were present only in Silybum marianum extract Table 1.
Flavonoids isolated from Plantago species were able to strongly inhibit the proliferation of human cancer cell lines Galvez et al. Percentage inhibition was calculated acitvity comparing the OD of control well with that ahticancer test sample: Cancer is the uncontrolled growth and division of cells and it leads to a mass of cells known as tumor. View at Google Scholar P.